南京文达化玻试剂有限公司(NanjingWendalabCo., Ltd.),致力于为生物、化学领域的基础研究、转化研究、以及规模化生产等多个领域提供可靠的一站式、多元化实验室产品解决方案。公司拥有一支由海外博士、硕士及本科生组成的素质精良的科研、销售及市场运作团队,团队成员在生物及癌症基础研究领域的多年深耕与发展为公司更好地理解科研工作者的实验需求、提供产品与精良服务奠定了夯实的基础。
南京文达化玻试剂有限公司主营业务包括化学试剂,生物试剂,玻璃制品,实验仪器,实验耗材,器械,宠物器械等,现已成为国内少数几家提供先进的微纳米仪器的企业。目前公司已为多家科研机构、高校、制药企业、食品、环境、医院、第三方检测、疾控多个行业和单位提供了多类别的实验室产品,并确立了长期合作关系。
公司于2021年成立同名品牌“Wendalab”,供应实验耗材、玻璃制品等产品。未来公司将不断完善管理体系、丰富供应渠道;本着服务科研、诚信经营的理念;坚守致敬科研、助力科研的使命为您的科研之旅保驾**。
Wendalab目前代理或经销的国内外品牌包括但不限于:西陇试剂、上海罗恩试剂、MERCK、Sigma、国药试剂、麦克林、百灵威、阿拉丁、天津科密欧、天津大茂;Cell Signaling Technology、Qiagen、Takara、abcam、Biorad、Santa cruz、BD、Abconal、Beyotime、Vazyme、梅里埃;葵花、蜀牛、天玻、博美、华欧;赛默飞世尔ThermoFisher、安捷伦Agilent、沃特世Waters、默克密理博Millipore、艾本德Eppendorf、大龙等;易必迪ibidi、康宁corning、杭州新星等。
RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This RPMI is modified as follows:
With | Without | ||
• L-glutamine | • HEPES | ||
• Phenol Red |
The complete formulation is available.
Using RPMI
RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Product use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP manufacturing and quality system
RPMI 1640 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical RPMI 1640 product made in our Scotland facility (21875-158). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.